Plant transformation: A pilus in Agrobacterium T-DNA transfer
نویسندگان
چکیده
Agrobacterium tumefaciens transfers a protein-DNA complex to plant cells in a process similar to bacterial conjugation; the mechanism of transfer is beginning to be unravelled by biochemical, genetic and electron microscopic studies.
منابع مشابه
Plant proteins that interact with VirB2, the Agrobacterium tumefaciens pilin protein, mediate plant transformation.
Agrobacterium tumefaciens uses a type IV secretion system (T4SS) to transfer T-DNA and virulence proteins to plants. The T4SS is composed of two major structural components: the T-pilus and a membrane-associated complex that is responsible for translocating substrates across both bacterial membranes. VirB2 protein is the major component of the T-pilus. We used the C-terminal-processed portion o...
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Agrobacterium tumefaciens is a phytopathogenic bacterium that causes crown gall disease by transferring transferred DNA (T-DNA) into the plant genome. The translocation process is mediated by the type IV secretion system (T4SS) consisting of the VirD4 coupling protein and 11 VirB proteins (VirB1 to VirB11). All VirB proteins are required for the production of T-pilus, which consists of processe...
متن کاملArabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation
Agrobacterium tumefaciens can genetically transform various eukaryotic cells because of the presence of a resident tumor-inducing (Ti) plasmid. During infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is transferred from bacteria into plant cells and causes plant cells to abnormally synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and several virulen...
متن کاملRole of Agrobacterium VirB11 ATPase in T-pilus assembly and substrate selection.
The VirB11 ATPase is a subunit of the Agrobacterium tumefaciens transfer DNA (T-DNA) transfer system, a type IV secretion pathway required for delivery of T-DNA and effector proteins to plant cells during infection. In this study, we examined the effects of virB11 mutations on VirB protein accumulation, T-pilus production, and substrate translocation. Strains synthesizing VirB11 derivatives wit...
متن کاملExtracellular VirB5 Enhances T-DNA Transfer from Agrobacterium to the Host Plant
VirB5 is a type 4 secretion system protein of Agrobacterium located on the surface of the bacterial cell. This localization pattern suggests a function for VirB5 which is beyond its known role in biogenesis and/or stabilization of the T-pilus and which may involve early interactions between Agrobacterium and the host cell. Here, we identify VirB5 as the first Agrobacterium virulence protein tha...
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ورودعنوان ژورنال:
- Current Biology
دوره 6 شماره
صفحات -
تاریخ انتشار 1996